| 基于岩羊（Pseudois nayaur）粪便DNA样本中AMEL基因的PCR扩增，进行性别鉴定 |
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| 编辑： 符琳琳 2015-09-16 | |
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在分子生态学和野生动物保护中利用无创性遗传采样来鉴定野生动物的性别是一个非常有价值和重要的工具。使用釉原蛋白基因进行性别鉴定已经在很多物种中得到应用，因为该方法只需要一对引物扩增X和Y连锁等位基因。然而，这种方法还没有被用于研究野外野生动物的粪便。 在这项研究中，利用已知性别岩羊的组织样本和粪便样本检测性别决定的有效性之后，研究人员使用釉原蛋白引物（SE47/SE48）这种方法对222份野生岩羊（Pseudois nayaur）粪便样品进行检测。该项研究表明，这种方法是检测岩羊是非常可靠的（80.2%）。釉原蛋白可以利用粪便样本来确定野生动物的性别。 详细内容如下： Sex identification based on AMEL gene PCR amplification from blue sheep (Pseudois nayaur) fecal DNA samples Liu X1, Yang YY1, Wang XM1, Liu ZS2, Wang ZH1, Ding YZ1 Abstract The use of noninvasive genetic sampling to identify the sex of wild animals is an extremely valuable and important tool in molecular ecology and wildlife conservation. Sex determination using the amelogenin gene has been conducted in many species because only a single pair of primers is required to amplify both X- and Y-linked alleles. However, this method has not been used in field research with the feces of wildlife. In this study, we applied this method to 222 fecal samples from wild blue sheep (Pseudois nayaur) using amelogenin primers (SE47/SE48) after testing the effectiveness of sex determination using tissue samples and fecal samples from blue sheep of known sex. We found this method to be highly reliable (80.2%) for blue sheep. Amelogenin can be used to identify the sex of wild animals using fecal samples. Keywords: Amelogenin, Fecal sample, Non-invasive genetic sample, Blue sheep, Polymerase chain reaction-based sex-typing 相关信息: |
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