国家肉羊产业技术体系 - 阿尔及利亚东北部流产母羊胎盘组织和冻结胎儿流产衣原体的血清阳性率和分子特征

阿尔及利亚东北部流产母羊胎盘组织和冻结胎儿流产衣原体的血清阳性率和分子特征

编辑：符琳琳 2015-11-16

母羊地方性流产在世界各地的羊群中是一个最严重的健康问题，它显著影响经济发展,因为流产使产奶量下降并产生弱羔。此外，细菌人畜共患。研究人员进行了一个横断面研究，利用C.特定流产间接ELISA试剂盒以确定在君士坦丁的552只母羊中与流产衣原体感染相关的血清阳性率和危险因素。研究人员应用PCR-限制性片段长度多态性（RFLP）和DNA测序在10个绵羊胎儿和8个胎盘中对衣原体DNA进行了研究。

研究发现，7.2%的母羊血清反应呈阳性，33.3%的母羊羊群至少有一个阳性母羊。相邻农场工人的拜访（或 =7.667，95% CI（或）=2.307；27.203）被定义为一个危险因素。弱羔的分娩（或 =2.920，95% CI（或）=1.022；8.342）和羔羊败血症（或 =9.971，95% CI（或）=2.383；41.713）与衣原体感染密切相关。六个胎儿和四个胎盘的C.流产PCR-RFLP分析显示出阳性信号。omp2基因测序发现96%阿尔及利亚菌株与 C.流产FAS菌株是相似的。C.流产在阿尔及利亚东北部发挥着主要作用。研究结果表明必须实施适当的控制措施，以减少经济损失和避免人为污染。

原文摘要如下：

Seroprevalence and molecular characterization of Chlamydia abortus in frozen fetal and placental tissues of aborting ewes in northeastern Algeria

Hireche S¹, Ababneh MM², Bouaziz O³, Boussena S³

Abstract: Enzootic abortion of ewes is one of the most serious health problems in sheep flocks worldwide. It has a significant economic impact because abortion, decrease in milk production and weak lambs. Besides, the bacteria is zoonotic. A cross-sectional study was conducted to determine the seroprevalence and risk factors associated with Chlamydia abortus infection in 552 ewes in Constantine using a C. abortus-specific indirect ELISA kit. Chlamydial DNA was investigated in ten ovine fetuses and eight placentas using PCR- restriction fragment length polymorphism (RFLP) and DNA sequencing. The study concluded that 7.2% of ewes were seropositive and 33.3% of sheep flocks had at least one seropositive ewe. Adjacent farmworker visits (OR=7.667, 95% CI (OR)=2.307; 27.203) was defined as a risk factor. Deliveries of weak lambs (OR=2.920, 95% CI (OR)=1.022; 8.342) and septicemia in lambs (OR=9.971, 95% CI (OR)=2.383; 41.713) were significantly associated with chlamydial infection. PCR-RFLP analysis revealed positive signals to C. abortus in six fetuses and four placentas. Sequencing of the omp2 gene revealed that the Algerian strain is 96 % similar with C. abortus FAS strain. C. abortus plays a major role in abortion in northeastern Algeria. Appropriate control measures must be implemented to reduce economic losses and to avoid human contamination.

Keywords: Algeria, Chlamydia abortus, DNA sequencing, Ewes, PCR-RFLP; Seroprevalence

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