2015年6 月加拿大阿尔伯塔自由放养的大角羊被诊断为患有恶性卡他热病。临死前和大体病理表现为肌肉萎缩，明显的体重减轻和双侧对称性脱毛，脸部、耳廓内侧面、背躯干、四肢远端、会阴部和尾部色素沉着，结痂。组织学上，由含有较多的多核巨细胞和较少的淋巴细胞和嗜酸性粒细胞的肉芽肿性壁毛囊炎对皮肤病变进行了表征并且这与先前的慢性绵羊疱疹病毒2（OvHV-2）感染的报道一致。多个皮肤样本的聚合酶链反应（PCR）对OvHV-2 DNA呈现阳性，病毒DNA部分序列分析发现与参考GenBank OvHV-2有94%的同源性。定量PCR证实在皮损组织OvHV-2 DNA水平增加。在排除其他疾病过程的基础上，肉眼和组织学病变，PCR和病毒DNA的测序结果，可用来确诊OvHV-2-调节恶性卡他热样皮炎。

原文摘要如下：

Sheep-Associated Malignant Catarrhal Fever-Like Skin Disease in a Free-Ranging Bighorn Sheep ( Ovis canadensis ), Alberta, Canada

Slater OM¹, Peters-Kennedy J², Lejeune M³, Gummer D⁴, Macbeth B^1,4, Warren A¹, Joseph T⁵, Li H^6,7, Cunha CW⁶, Duignan PJ^1,3

Abstract：Malignant catarrhal fever-like clinical disease was diagnosed in a free-ranging bighorn sheep ( Ovis canadensis ) from Alberta, Canada, in June 2015. Antemortem and gross pathology findings included muscle atrophy, marked weight loss, and bilaterally symmetric alopecia with hyperpigmentation and crusting over the face, medial surfaces of the pinnae, dorsal trunk, distal limbs, perineal area, and tail. Histologically, the skin lesions were characterized by granulomatous mural folliculitis with numerous multinucleated giant cells and fewer lymphocytes and eosinophils consistent with previous reports of chronic ovine herpesvirus-2 (OvHV-2) infection. Multiple skin samples were positive for OvHV-2 DNA on polymerase chain reaction (PCR), and on partial sequencing of the viral DNA, there was 94% homology with reference GenBank OvHV-2. Quantitative PCR confirmed an increased level of OvHV-2 DNA in the lesional skin tissues. Based on exclusion of other disease processes, gross and histological lesions, PCR, and viral DNA sequencing results, a diagnosis of OvHV-2-mediated malignant catarrhal fever-like dermatitis was made.

Keywords: Alberta, Canada, Ovis Canadensis, bighorn sheep, dermatitis, malignant catarrhal fever, ovine herpesvirus-2, skin

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