| 在自然感染的舍饲绵羊肺组织中肺腺癌的诊断和发病率 |
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| 编辑： 符琳琳 2016-07-21 | |
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本研究旨在检测影响舍饲羊场的绵羊肺部疾病即绵羊肺腺癌（OPA）。在共75只自然死亡绵羊的剖检中彻底地检测了OPA病变。无菌采集每只绵羊受影响的部分肺组织切片并分为两部分进行检测；聚合酶链反应（PCR）检测和组织病理学检测。 对肺组织PCR检测发现，6只绵羊（8%）的JSRV呈现阳性。他们其中2只为3-6月龄并没有表现出临床症状和肉眼可见的OPA病变。4只PCR呈阳性成年羊在大体和组织病理学检查中显示出OPA的特征性病变。在已知感染JSRV的特异性抗体反应的情况下，没有进行有效的血清学试验诊断。本研究采用基于病毒的长末端重复JSRV的U3区的序列引物PCR方法对肺组织进行分析有助于绵羊OPA的潜伏期和发病期的筛选。 原文摘要入下： Diagnosis and prevalence of ovine pulmonary adenocarcinoma in lung tissues of naturally infected farm sheep Sonawane GG1, Tripathi BN2, Kumar R3, Kumar J1 Abstract: This study was aimed to detect ovine pulmonary adenocarcinoma (OPA) in sheep flocks affected with pulmonary disorders at organized farm.A total of 75 sheep died naturally were thoroughly examined for the lesions of OPA during necropsy. Tissue sections from affected portion of the lungs from each animal were collected aseptically and divided into two parts; one each for polymerase chain reaction (PCR) and another for histopathology.On PCR examination of lung tissues, six sheep (8%) were found to be positive for JSRV. Two of them were 3-6 months of age and did not show clinical signs/gross lesions of OPA. Four adult sheep positive on PCR revealed characteristic lesions of OPA on gross and histopathological examination.In the absence of known specific antibody response to the infection with JSRV, there is no diagnostic serological test available. The PCR assay employed in this study on lung tissues, using primers based on the U3 region of the viral long terminal repeat for JSRV would be helpful in the screening of preclinical and clinical cases of OPA in sheep. Keywords: diagnosis, lung tissues, ovine pulmonary adenomatosis, sheep 相关信息:
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